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现在位置首页>实验试剂制品>文库及构建>cDNA文库>Hieff NGS® Complete Adapter Kit for MGI® 13360ES04
Hieff NGS® Complete Adapter Kit for MGI® 13360ES04
Hieff NGS® Complete Adapter Kit for MGI® 13360ES04
  • Hieff NGS® Complete Adapter Kit for MGI® 13360ES04

Hieff NGS® Complete Adapter Kit for MGI® 13360ES04

产品报价:1153元

更新时间:2019/8/23 8:47:52

地:上海

牌:翊圣生物

号: 13360ES04

厂商性质: 生产型,贸易型,服务型,

公司名称: 上海翊圣生物科技有限公司

产品关键词: MGI高通量文库构建   DNA Adapter   Hieff NGS® Complete Adapter Ki  

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翊圣生物 : (13916792673) (400-6111-883)

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Hieff NGS? Complete Adapter Kit for MGI?

产品信息

产品名称

产品编号

规格

价格(元)

Hieff NGS? Complete Adapter Kit for MGI?, Set 1

13360ES04

16×4 T

1153.00

13360ES16

16×16 T

3653.00

13360ES96

16×100T

15653.00

Hieff NGS? Complete Adapter Kit for MGI?, Set 2

13361ES04

16×4 T

1153.00

13361ES16

16×16 T

3653.00

13361ES96

16×100T

15653.00

Hieff NGS? Complete Adapter Kit for MGI?, Set 3

13362ES04

16×4 T

1153.00

13362ES16

16×16 T

3653.00

13362ES96

16×100T

15653.00

Hieff NGS? Complete Adapter Kit for MGI?, Set 4

13363ES04

16×4 T

1153.00

13363ES16

16×16 T

3653.00

13363ES96

16×100T

15653.00

Hieff NGS? Complete Adapter Kit for MGI?, Set 5

13364ES04

16×4 T

1153.00

13364ES16

16×16 T

3653.00

13364ES96

16×100T

15653.00

Hieff NGS? Complete Adapter Kit for MGI?, Set 6

13365ES04

16×4 T

1153.00

13365ES16

16×16 T

3653.00

13365ES96

16×100T

15653.00


Hieff NGS? Complete Adapter Kit for MGI?是针对MGI高通量文库构建专用配套试剂盒,共分为6个Set,每个Set中包含16种不同的Barcode接头适用于MGI高通量测序平台多样品DNA文库构建。试剂盒中提供的所有试剂都经过严格的质量控制和功能验证,最大程度保证了文库构建的稳定性和可重复性。产品描述

产品组分

编号

组分

产品规格

16×4 T

16×16 T

16×100 T

13360

DNA Adapter 1-16

20 μL each

80 μL each

500μL each

13361

DNA Adapter 17-32

20 μL each

80 μL each

500μL each

13362

DNA Adapter 33-48

20 μL each

80 μL each

500μL each

13363

DNA Adapter 49-64

20 μL each

80 μL each

500μL each

13364

DNA Adapter 65-80

20 μL each

80 μL each

500μL each

13365

DNA Adapter 81-96

20 μL each

80 μL each

500μL each

运输和保存方法

冰袋运输,-20℃保存,有效期1年。

 

注意事项

1)本试剂盒中DNA Adapter的浓度统一为10 μM,单个文库构建的接头使用量根据所用试剂盒进行调整。

2)若有使用 MGI 其它建库试剂盒中的序号为 501-596 的接头,由于设计工艺不同,禁止混用,否则数据无法拆分。

3)切勿加热接头,应在室温让其缓慢溶解,实验室温度最好设置为20-25℃。接头避免反复冻融,可短暂存放于4℃。

4)小规格试剂盒(16×4 T每种组分的DNA Adapter包装量足够进行64DNA文库构建,中规格试剂盒(16×16 T)每种组分的DNA Adapter包装量足够进行96DNA文库构建,大规格试剂盒(16×100 T)每种组分的DNA Adapter包装量足够进行1600DNA文库构建。

5)使用Hieff NGS? Complete Adapter Kit for MGI?试剂盒构建的DNA文库结构如下:

   5’ - Ad153Ω_Bottom_ 2 - Insert DNA Sequence - Ad153_5T_1-index- 3’

6)试剂盒中提供的每种DNA Adapter中都包含Ad153_5T_1-index,且提供一种Barcode序列标签,用于高通量测序时区分样品。

7)为了您的安全和健康,请穿实验服并戴一次性手套操作。

使用方法

DNA Adapter的序列如下

Ad153Ω_Bottom_ 25’-TTGTCTTCCTAAGGAACGACATGGCTACGATCCGACTT-3’

Ad153_5T_1-index5’-AGTCGGAGGCCAAGCGGTCTTAGGAAGACAANNNNNNNNNN1CAACTCCTTGGCTCACA-3’

1NNNNNNNNNN表示接头的Barcode区域,其在每个接头中的序列见下表。测序前在Sample Sheet中只需输入这个Barcode序列(10 bp)即可。

名称

序列

 

名称

序列

Ad153_5T_1-index1

TAGGTCCGAT

 

Ad153_5T_1-index49

ATGTACGCAG

Ad153_5T_1-index2

GGACGGAATC

 

Ad153_5T_1-index50

TTAATGAGAT

Ad153_5T_1-index3

CTTACTGCCG

 

Ad153_5T_1-index51

TGCGCCACTT

Ad153_5T_1-index4

ACCTAATTGA

 

Ad153_5T_1-index52

CATTAAGGCC

Ad153_5T_1-index5

TTCGTATCCG

 

Ad153_5T_1-index53

CCGCCTCAGA

Ad153_5T_1-index6

GGTAACGAGC

 

Ad153_5T_1-index54

AATCGGCTCG

Ad153_5T_1-index7

CAACGTATAA

 

Ad153_5T_1-index55

GCCGGTTATC

Ad153_5T_1-index8

ACGTCGCGTT

 

Ad153_5T_1-index56

GGAATATTGA

Ad153_5T_1-index9

TTCTGCTAGC

 

Ad153_5T_1-index57

ATTCAACGGA

Ad153_5T_1-index10

AGGAAGATAG

 

Ad153_5T_1-index58

AACTGTACTG

Ad153_5T_1-index11

GCTCTTGCTT

 

Ad153_5T_1-index59

GTACCTCAAT

Ad153_5T_1-index12

CAAGCACGCA

 

Ad153_5T_1-index60

GACTTCTAAT

Ad153_5T_1-index13

CGGCAATCCG

 

Ad153_5T_1-index61

TGAAGCGTTG

Ad153_5T_1-index14

ATCAGGATTC

 

Ad153_5T_1-index62

CGTGCGATCC

Ad153_5T_1-index15

TCATTCCAGA

 

Ad153_5T_1-index63

TCGGAAGGCA

Ad153_5T_1-index16

GATGCTGGAT

 

Ad153_5T_1-index64

CCGATGTCGC

Ad153_5T_1-index17

GTGAGTGATG

 

Ad153_5T_1-index65

ACTTAGAATG

Ad153_5T_1-index18

GAGTCAGCTG

 

Ad153_5T_1-index66

TCCAAGCCTG

Ad153_5T_1-index19

TGTCTGCGAA

 

Ad153_5T_1-index67

AGACGATGAT

Ad153_5T_1-index20

ATTGGTACAA

 

Ad153_5T_1-index68

CTCACAAGAC

Ad153_5T_1-index21

CGATTGTGGT

 

Ad153_5T_1-index69

CGTTCCTACT

Ad153_5T_1-index22

ACAGACTTCC

 

Ad153_5T_1-index70

GTGGTTGTGA

Ad153_5T_1-index23

TCCACACTCT

 

Ad153_5T_1-index71

GAAGGCCTGC


Ad153_5T_1-index24

CACCACAAGC

 

Ad153_5T_1-index72

TAGCTTGCCA

Ad153_5T_1-index25

TAGAGGACAA

 

Ad153_5T_1-index73

GACAATGCTC

Ad153_5T_1-index26

CCTAGCGAAT

 

Ad153_5T_1-index74

GCTAATCACA

Ad153_5T_1-index27

GTAGTCATCG

 

Ad153_5T_1-index75

AGTCCATAGG

Ad153_5T_1-index28

GCTGAGCTGT

 

Ad153_5T_1-index76

CTATCGCCTA

Ad153_5T_1-index29

AACCTAGATA

 

Ad153_5T_1-index77

ATCGTGGTCT

Ad153_5T_1-index30

TTGCCATCTC

 

Ad153_5T_1-index78

TGGCTAATAC

Ad153_5T_1-index31

AGATCTTGCG

 

Ad153_5T_1-index79

CAGTGCAGAG

Ad153_5T_1-index32

CGCTATCGGC

 

Ad153_5T_1-index80

TCAGGCTGGT

Ad153_5T_1-index33

GCAACGATGG

 

Ad153_5T_1-index81

ATACTCACGC

Ad153_5T_1-index34

TAATCGTTCA

 

Ad153_5T_1-index82

ATGCTCCGCG

Ad153_5T_1-index35

GTTCGCTCTA

 

Ad153_5T_1-index83

TGTGAACTTG

Ad153_5T_1-index36

TCTCACACAT

 

Ad153_5T_1-index84

GAGAGGTGCT

Ad153_5T_1-index37

CTGTTAGGAT

 

Ad153_5T_1-index85

TGCACTGTAA

Ad153_5T_1-index38

CGCAGACGCG

 

Ad153_5T_1-index86

GCCTAGGCAA

Ad153_5T_1-index39

AAGGATCATC

 

Ad153_5T_1-index87

CCATCATAGC

Ad153_5T_1-index40

AGCGTTGAGC

 

Ad153_5T_1-index88

CATGGTAATT

Ad153_5T_1-index41

TTAGATGCAT

 

Ad153_5T_1-index89

CACCATGTCT

Ad153_5T_1-index42

GTCCAGAGCT

 

Ad153_5T_1-index90

ATATGTCTGG

Ad153_5T_1-index43

CACGTGATAG

 

Ad153_5T_1-index91

AAGGAAGCGT

Ad153_5T_1-index44

CCACTAGTCC

 

Ad153_5T_1-index92

TCAAGACGTC

Ad153_5T_1-index45

TGGACTTGGC

 

Ad153_5T_1-index93

CCGCTCAGTA

Ad153_5T_1-index46

GCTTGACAGG

 

Ad153_5T_1-index94

GGTGTGTACA

Ad153_5T_1-index47

AAGACCTCTA

 

Ad153_5T_1-index95

TTCACGTAAG

Ad153_5T_1-index48

AGTTGCCATA

 

Ad153_5T_1-index96

GGTTCCACAC

推荐Barcode组合

4Adapter 成组:1-4,13-16

8Adapter 成组:41-48

 

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多重PCR定制咨询


 

HB190809

 


上海翊圣生物科技有限公司

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手机:13916792673

联系人:翊圣生物

电话:400-6111-883

传真:021-34615995

QQ:4006111883

(联系我时,请说明是在大发3d上看到的,谢谢!)